Ecological implications of an improved direct viable count method for aquatic bacteria
نویسندگان
چکیده
منابع مشابه
Ecological implications of an improved direct viable count method for aquatic bacteria.
The direct viable count method first described by Kogure et al. (Can. J. Microbiol. 25:415-420, 1979) was improved by using an antibiotic cocktail instead of nalidixic acid alone. We screened 100 marine isolates from two coastal areas for their sensitivities to five replication-inhibiting antibiotics, including four quinolones (nalidixic, piromidic, and pipemidic acids and ciprofloxacin) and on...
متن کاملComparison of two direct-count techniques for enumerating aquatic bacteria.
Planktonic bacteria from an estuary were concentrated on membrane filters and counted with both a scanning electron microscope and an epi-illuminated fluorescent microscope. Counts on 0.2 micron Nuclepore filters (polycarbonate) were significantly higher (P less than 0.001) than counts on 0.2-micron Sartorius filters (cellulose). In contrast, there was not a statistically significant difference...
متن کاملImproved direct viable count procedure for quantitative estimation of bacterial viability in freshwater environments.
A direct viable count (DVC) procedure was developed which clearly and easily discriminates the viability of bacterial cells. In this quantitative DVC (qDVC) procedure, viable cells are selectively lysed by spheroplast formation caused by incubation with antibiotics and glycine. This glycine effect leads to swollen cells with a very loose cell wall. The viable cells then are lysed easily by a si...
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چکیده ندارد.
ERA: Suitable Method for Estimation of Ecological Effects of Pesticide Contamination on Aquatic Species
An ecological risk assessment (ERA) was conducted for Shadegan Wetland in Iran. The objective of this study was to assess the risk to phytoplankton, zooplankton, invertebrate, insect larvae, and fish affected by pesticide in Shadegan Wetland. Five pesticides (DDT, Aldrine, Dieldrin, Ametryn and Lindane) were assessed to evaluate the impact to aquatic community in the Shadegan Wetland. The risk ...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1997
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.63.9.3643-3647.1997